ABSTRACT
The rst Klun and Debboun module (K & D module) was designed for in vivo experimental use on humans1 to evaluate the mosquito-feeding deterrent efcacy of chemicals that were toxicologically safe for application on the skin of humans. Impetus for development of this bioassay system resulted from problems with experimental techniques developed and used on humans between 1983 and 1992. These methods often required lengthy periods for observation and reducing the number of replications. The physical designs of the apparatus, where mosquitoes comingled in a common area and might have switched between feeding areas, made the data multinomial and induced correlations, making them more difcult to correctly analyze.
