ABSTRACT

CONTENTS List of Abbreviations ................................................................ 328 9.1 Introduction ..................................................................... 328

9.1.1 Aphid Biology ....................................................... 328 9.1.2 From the Cytogenetic to the Genomic

Analyses ................................................................ 329 9.1.3 From Genome Size to Genome

Structure ................................................................332 9.2 Protocols .......................................................................... 334

9.2.1 Laboratory Equipment .......................................... 334 9.2.2 Aphid Breeding ......................................................335 9.2.3 Source of Chromosomes and Chromosome

Slide Preparations ..................................................335 9.2.3.1 Spreading Preparation ............................. 336 9.2.3.2 Squash Preparation .................................. 336 9.2.3.3 Giemsa Staining .......................................337 9.2.3.4 Propidium Iodide Staining .......................337