ABSTRACT
CONTENTS List of Abbreviations .................................................................. 95 4.1 Introduction ....................................................................... 96
4.1.1 Anopheles gambiae as a Disease Vector ................. 97 4.1.2 Cytogenetics of Anopheles gambiae ....................... 98 4.1.3 Genome Assembly of Anopheles gambiae ............101 4.1.4 Aedes aegypti as a Disease Vector ........................ 102 4.1.5 Cytogenetics of Aedes aegypti .............................. 104 4.1.6 Genome Assembly of Aedes aegypti......................105 4.1.7 Culex quinquefasciatus as a Disease Vector .........105 4.1.8 Cytogenetics of Culex quinquefasciatus ............... 106 4.1.9 Genome Assembly of Culex quinquefasciatus ..... 107
4.2 Protocols .......................................................................... 108 4.2.1 Fluorescent In Situ Hybridization with Polytene
Chromosomes of Malaria Mosquitoes .................. 108 4.2.1.1 Materials for Fluorescent In Situ
Hybridization with Polytene Chromosomes .......................................... 108
4.2.1.2 Dissection of Ovaries from HalfGravid Anopheles Females .......................111
