ABSTRACT

Since its early applications in the 1970s, genetic engineering (or recombinant DNA technology) has become one of the principal technological advances in modern biotechnology. Genetic engineering allows selected individual genes to be transferred from one organism into another and also between nonrelated species. Organisms derived from recombinant DNA technology are termed genetically modified organisms (GMOs), and these are defined as those organisms in which the genetic material has been altered in a way that does not occur naturally by mating or natural recombination (WHO 2002). Recombinant DNA technology has become valuable for the experimental investigation of many aspects of plant biochemistry and physiology that cannot be addressed easily by any other experimental means (Wisniewski et al. 2002). This technology offers an extraordinary opportunity to investigate the molecular mechanisms of important processes, such as plantmicrobe interactions, development, response to abiotic and biotic stress, and signal transduction

CONTENTS

2.1 Introduction ............................................................................................................................ 15 2.2 Challenges in GMO Analysis ................................................................................................. 17

2.2.1 GMO Detection in the Food Supply Chain ................................................................ 17 2.2.2 Safety Evaluation of GMOs ........................................................................................ 18

2.3 Foodomics Applications in GMO Analysis ............................................................................ 18 2.3.1 Comprehensive GMO Screening ................................................................................ 19 2.3.2 Comparative Compositional Analysis of GMOs ........................................................20