ABSTRACT

States of Oligomers ........................................................................... 247 11.3.4 Measuring the Labeling Probability Using SpIDA ..........................250 11.3.5 Measuring Three Oligomer Populations in Single Images

Including Mislabeling ....................................................................... 251 11.4 Conclusion .................................................................................................... 252 Acknowledgments .................................................................................................. 253 Appendix A: Theoretical Basis of SpIDA .............................................................. 253

Two-Oligomer-Population Mixture ..............................................................254 Three-Oligomer-Population Mixture ............................................................ 255 Mislabeling of Subunits in Higher-Order Oligomers ................................... 255 Analog Detector Calibration ......................................................................... 255 Materials and Methods .................................................................................256