ABSTRACT
Chapter 2 described the Michaelis-Menten mechanism of the enzymatic reaction:E + S ES E + P The overall reaction kinetics tells that the maximum rate is equal to k+2e0. Thus, we can easily determine the rate constant k+2dividing the maximum rate by the total enzyme concentration. However, it is not certain whether the rate constant is correct without observing directly the step: ES to E + P. As described in Chapter 2 (Section 2.3.2), the time scale of the step is usually below 1 sec. Therefore, the special apparatus is required to observe the step. The readers may recall one of the stopped-flow methods in Chapter 2. This chapter describes the method in detail and focuses on the analysis of the first-order reaction.
