ABSTRACT
The size of the nanoparticles affects the biodistribution and clearance of the nanoparticles in vivo [14,16]. NPs with a size range of 100-300 nm are ideal to achieve tumor targeting as a consequence of EPR effect [17]. Given the importance of nanoparticle size in tumor targeting, particle size and polydispersity index (PDI) of the nanoparticles were monitored to ensure uniform size distribution of the formulation [18]. The particle size and PDI of the nanoparticles were found to be sensitive to various formulation and process parameters, including polymer concentration, ratio of the antisolvent and the solvent, and pH of the solvent [19].
