ABSTRACT
Neuroproteomics enables the holistic interrogation of functional changes down at the molecular level following brain injury. Results are a window into the protein-driven mechanisms underlying cellular and anatomical degradation as well as regeneration. In this chapter, we discuss the merits and limitations of neuroproteomics in the context of studying brain injury, and discuss important consideration for study design. We present results from our recent studies on how this new level of detail informs our understanding of the fundamental processes governing regeneration. Placed in context with observed anatomical restructuring, neuroproteomic results can be instrumental in guiding targeted interventions of regeneration. Delineating the temporal series of molecular events would facilitate time-dependent therapeutic administration as well as precision endpoints with which to decipher efficacy. Ultimately, deep-neuroproteomic analysis can enhance our understanding of the complex interactive-nature of the molecular underpinnings of brain injury pathobiology and help delivery on the promise of improved care and treatment.
