ABSTRACT
The genome projects provided us with a huge amount of information at the DNA level and lead to the identication of thousands of open reading frames. The demand for technologies allowing the functional analysis of gene products is, therefore, dramatically increased. Discovery and characterization of interacting gene products, molecular recognition, and molecular modeling became central to life sciences. Surface display technology based on two pivotal concepts-physical linkage between genotype and phenotype and rescue of individual clones from large libraries by afnity selection-has the potential to substantially contribute to functional genomics. The expansion of surface display technology in biosciences is facilitated by the adaptability of the systems to high-throughput screening formats for automated library handling. While recombinant DNA techniques allow construction of highly
CONTENTS
12.1 Overview ....................................................................................................... 295 12.2 Introduction ..................................................................................................296 12.3 Cloning Vectors ............................................................................................296 12.4 Display of cDNA Libraries on Phage Surface .............................................. 298 12.5 Problems Associated with the Display of cDNA Libraries on Phage Surface ...302 12.6 Adaptability of Phage Display to High-Throughput Screening Technology ...303 12.7 Conclusions ...................................................................................................304 Acknowledgments ..................................................................................................304 References ..............................................................................................................305
