ABSTRACT
Freeze-thaw in liquid nitrogen three times. e thaw
steps will take about 10 minutes each; you may proceed
to the next step while you are waiting.
1. Pipette internal and external solutions into the black
plate as shown in Figure 17.1.
Freeze-thaw in liquid nitrogen three times. e thaw
steps will take about 10 minutes each; you may proceed
to the next step while you are waiting.
1. Pipette internal and external solutions into the black
plate as shown in Figure 17.1.