ABSTRACT

These steps are essentially the same as in cloning any particular DNA; and since the vectors appropriate for different types of libraries were summarized in Table 2.2 in Chapter 2, and cloning procedures described in Chapter 2, this chapter will only cover the basic principles and areas of application. (Please refer to special laboratory manuals and published articles for technical details of library construction.)

The essential difference from ordinary cloning (for sequencing or for expression, and so forth, purposes) is that the library must include up to millions of different bacteria, each of which has a different DNA fragment. Therefore, the transformation efficiency and representation/ coverage become ever more important.