ABSTRACT
Original sequences obtained from a sequencing machine require editing and creation of a consensus sequence. Sequencing machines of such manufacturers as Applied Biosystems and Amersham MegaBase provide the output fi les containing image information in a mode of peaks (chromatograms) and their interpretation as letters for each of four main nucleotides C, T, A and G; they may be supplemented sometimes by the letter N, if interpretation is not clear, or by some other letters if it is ambiguous. Different software editors allow conversion of this information into another fi le that represents the digital letter sequence of corresponding nucleotides in the gene or its section. However, this information is not quite suitable for quantitative comparison of this and other sequences and for phylogenetic analysis. To be ready for such analysis sequences must be carefully edited. Different software processors allow conversion of edited, consensus sequences into another fi le that will represent the digital letter sequence of corresponding nucleotides in the gene or its section for further comparisons. Many requirements for the sequence processing are met by such program packages (PP) as MEGA-4 or MEGA-5 (https://www.megasoftware.net/), BioEdit (https://www.mbio.ncsu.edu/BioEdit/bioedit.html), DAMBE (https://dambe.bio. uottawa.ca/dambe.asp.), etc.; at least 30 are available nowadays from a web-list (https:// evolution.genetics.washington.edu/phylip/software.html#methods). A very suitable PP tool for the primary editing is Chromas (Chromas-pro, https://www.fl u.org.cn/en or https://www.technelysium.com.au/chromas.html). Our working Chromas version (Chromas-pro 2.31) lets us perform all necessary operations for sequence editing:
• Opens chromatogram fi les from Applied Biosystems and Amersham MegaBace DNA sequencers.
