ABSTRACT
An example of a case in which the camera dynamic range is too low for proper acquisition of the specimen image is presented in Figure 1. In this Figure the sequence of images, acquired with different exposure time t, of the same cell nuclei is presented. These images were obtained with the usage of fluorescence microscopy and DAPI fluorescent dye, which is used for detection of DNA. Hence, in this case, the signal intensity is related to the amount of DNA contained in different cell sites. As it can be seen the dynamic range of object signal is too high for the used camera. Therefore, there is no possibility of acquisition of a single, proper exposed, image on which, both, low and high object signals are properly reproduced.
