Swine vesicular disease virus (SVDV) belongs to the Enterovirus genus within the family Picornaviridae. It causes an infectious and contagious disease in pigs called swine vesicular disease (SVD), characterized by the appearance of vesicles on the coronary bands of the feet, heels, skin, snout, tongue, lips, and teats, accompanied by fever. Its importance for animal health authorities relies in the fact that these signs are indistinguishable from those caused by other vesicular diseases of viral etiology affecting swine, including foot-andmouth disease (FMD), vesicular stomatitis (VS), and vesicular exanthema of swine (VES), and therefore, differential diagnosis in the laboratory is essential to discern between them.