ABSTRACT
Interestingly, while members of the genus Lyssavirus (e.g., rabies virus) replicate exclusivelyin mammals, viruses belonging to the genera Vesiculovirus and Ephemerovirus (e.g., bovine ephemeral fever virus) occur in both vertebrate and invertebratehosts. The agents of the genus Novirhabdovirus are present in sh and other aquatic animals, including
42.1 Introduction ..................................................................................................................................................................... 369 42.1.1 Classication ........................................................................................................................................................ 369 42.1.2 Morphology and Genome Organization .............................................................................................................. 370 42.1.3 Replication and Epidemiology ............................................................................................................................. 370 42.1.4 Clinical Features and Pathogenesis ..................................................................................................................... 371 42.1.5 Diagnosis ............................................................................................................................................................. 372
42.1.5.1 Virus Isolation ....................................................................................................................................... 372 42.1.5.2 Serological Detection of Viral Antigens and Antibodies ..................................................................... 372 42.1.5.3 RNA Detection ..................................................................................................................................... 372
42.1.6 Treatment and Prevention .................................................................................................................................... 372 42.2 Methods ........................................................................................................................................................................... 373
42.2.1 Sample Preparation .............................................................................................................................................. 373 42.2.1.1 Sample Collection ................................................................................................................................. 373 42.2.1.2 Virus Isolation ....................................................................................................................................... 373 42.2.1.3 RNA Isolation ....................................................................................................................................... 373
42.2.2 Detection Procedures ........................................................................................................................................... 373 42.2.2.1 Multiplex RT-PCR for the Detection of Vesicular Disease Viruses ..................................................... 373 42.2.2.2 Real-Time RT-PCR for the Detection of VSV-IN and VSV-NJ ........................................................... 373 42.2.2.3 RT-PCR and Enzyme Digestion for the Detection of VSV-IN and VSV-NJ ........................................ 374
42.3 Conclusion ....................................................................................................................................................................... 374 References ................................................................................................................................................................................. 374
invertebrates, and those of the genera Cytorhabdovirusand Nucleorhabdovirus are plant pathogens.
