ABSTRACT
Abstract Proteins are the most abundant bio-molecules found in living cells. The role of individual proteins and their interaction with other proteins are crucial for several cellular processes. Protein interacting with protein, DNA and ligand often drives the fate of various biochemical processes. There have been several efforts to annotate the individual protein functionality and its role in performing cellular processes. The impairment of protein functions can lead to an array of diseases and disorders in biosystems. With the advent of the post-genomic era there exists a large class of proteins with their functions still unknown. Around 150 genomes that are recently sequenced are yet to be assigned their physiological function and almost 30-50% of the genes are still having undefined functional characterization for their encoded proteins. Such class of proteins is termed as hypothetical proteins. A hypothetical protein is generally predicted to be expressed from ORFs (Open Reading Frame) with no experimental evidence of translation. These proteins constitute a considerable fraction of the proteomes of most of the organisms. Through the domain extraction of these proteins, it can be helpful to search and reveal many gene coding proteins, their structure and function. In order to understand the biological system of these species, their functional characterization of proteins is very important.
