ABSTRACT
A testing model for the detection and evaluation of multiple doses of neutrophil agonists and antagonists is described. Incubations are performed in microtiter plates, where oxidative metabolism is measured as superoxide anion production and in the same assay system the adhesion is measured as activity of acid phosphatase. The test is simple to handle and is highly reproducible, and, therefore, it appears particularly useful for drug screening. Dose-response curves of various cell stimulants and inhibitors showed that the lowest effective doses ranged between 10-6 and 10-10 M according to the agents employed. Adhesion appeared to be more sensitive to low doses than superoxide production.
