ABSTRACT
Manipulated gametes IVF and ICSI involve ovarian stimulation with collection of several oocytes, using drug regimens of variable complexity (see Chapter 14). The oocytes are then either placed together with sperm (IVF) or injected with sperm (ICSI). The gametes and consequent embryos are cultured, in specified culture media, usually for 2 days prior to embryo transfer, which may be changed and refreshed if culture is continued through to the blastocyst stage before embryo transfer on day 5.
