ABSTRACT
The hybridization reaction As double-stranded DNA is heated, a temperature is reached at which the two reaction strands separate. This process is called denaturation. The temperature at which half of the DNA molecules have denatured is called the melting temperature or Tm for that DNA. If the temperature is now lowered and falls below the T
will form hydrogen bonds with each other once more to re-form a double-stranded molecule. This process is called renaturation (or reannealing).
