Single-molecule localization microscopy (SMLM) methods such as stochastic optical reconstruction microscopy (Rust et al. 2006) (STORM) and photoactivated localization microscopy (Betzig et al. 2006) (PALM) have quickly been adopted by many laboratories as a reliable method for achieving optical resolution beyond the diraction limit. One reason for this rapid dissemination is that the method requires a fairly simple microscope setup, but perhaps more importantly, SMLM methods invite contributions from a variety of disciplines in chemistry and physics, as well as the ultimate applications of SMLM in cell biology. Probe design, labeling strategies, instrumentation development, and image analysis are all part of the process, making SMLM a truly interdisciplinary endeavor.