Super-Resolution Fluorescence Microscopy of the Nanoscale Organization of RNAP and DNA in E. coli

Cellular processes in bacteria are orchestrated by the spatiotemporal organization of proteins into functional units. e size and composition of such functional units is sensitively regulated in the bacterial cell, and among other factors depend on the cell cycle stage as well as external stimuli or growth conditions. Revealing parameters such as size, protein content, stoichiometry, and dynamics is crucial to understand the functionality of molecular machines, as well as their regulation at the molecular level.