ABSTRACT
Although in the past 4 years, several reviews appeared (Crossman et al. 2015b, Kohl et al. 2013, Soeller and Baddeley 2013) that highlight the enormous potential of super-resolution microscopy in cardiac imaging in general, experimental studies have been limited and centered around structural imaging in xed cells or tissue sections. Most attention has been devoted to structural imaging of Ca2+- channel-related structures and proteins (including one live-cell study), such as ryanodine receptor isoform 2 (RyR2), T-tubuli (TT [membrane invaginations]), caveolin-3 (CAV3), and junctophilin-2 (JPH2). Several xed-cell studies investigated the Na+ and ATP-sensitive K+ channel (NaV1.5, KATP) and their relation with structures such as connexin-43 (CX43), N-cadherin, and plakophilin-2 (PKP2) in the intercalated disc (ID) of the cardiomyocyte. Remaining work focuses on imaging a diversity of cardiomyocyte structures, such as mitochondria, CX43 and PKP2, α-actinin, actin, titin/myosin, and TT/CAV3/JPH2 in xed cardiomyocytes. e latter study also partly uses live-cell imaging. All these papers will be discussed below.
