Campeche is a state located in the southeast of the Gulf of Mexico and has a great variety of microclimates and native vegetation. Backyard orchards in rural communities in this state provide both introduced and native host fruits for Anastrepha spp. fruit flies, such as the “Kaniste” fruit (Pouteria campechiana) (Sapotacea). In this study, we sampled Kaniste fruits from backyards located in the municipality of Escarcega, Campeche, Mexico. Three species of the genus Anastrepha were recorded feeding on Kaniste fruits: Anastrepha fraterculus, Anastrepha serpentina, and Anastrepha hamata. These findings contribute to our knowledge of the hosts and diversity of the genus Anastrepha in Campeche, Mexico.