ABSTRACT
Monitorization of biological fluids and tissues to find relevant biomarkers from the epidemiological point of view could identify potential subpopulations to suffer adverse health effects. In the analytical procedures to determine xenobiotics in milk, liquid–liquid extraction using non-polar solvents is one the most used extraction technique. In the determination of polar organic compounds, liquid chromatography is mostly used since it allows the separation of a broad spectrum of compounds as far as polarity is concerned, which can be interesting in the analysis of emerging compounds and their metabolites and/or transformation products. The analytical method was validated at two concentration levels after spiking bovine whole milk samples with the target analytes at 10 and 40 ng/g. The apparent recoveries were determined by two different strategies: surrogate correction and matrix-matched calibration. Absolute recoveries were determined as the ratio of the concentrations calculated from the external calibration and the spiked concentration.
