Characterization of crude extract of protease from bacillus megaterium TR-10 as efforts to support halal collagen production process from waste fish

The purpose of this study is to determine the characteristics of crude protease extract from Bacillus megaterium TR-10, by determining the type of protease based on pH and the addition of EDTA, determining the effect of temperature, and the effect of adding metal ions (Fe²⁺, Mn²⁺, Mg²⁺, Zn²⁺, Cu²⁺) on protease activity, and its selectivity toward milkfish waste. The results showed that crude protease extract had neutral proteases, basic proteases, metalloproteases, and proteases that were inhibited by metal ions. Their activity increased from 37°C to 40°C, also in the presence of Mn²⁺ and Mg²⁺ ions, but the activity decreased in presence of Fe²⁺ ions, and even lost activity on addition of Zn²⁺ and Cu²⁺. The crude protease extract has selectivity toward various parts of milkfish waste with the order of scales > skin > bone > tail. Results show that the crude protease extract from B. megaterium TR-10 has the potential to replace porcine pepsin in the process of extracting collagen from milkfish waste.