ABSTRACT
Artificial insemination (AI) using sexed semen is the technology applied to obtain the sex offspring as expected. However, the concentration of sexed frozen semen has lower number than non-sexed frozen semen. This study aimed to evaluate the proportion of fetal sex resulting from artificial insemination using frozen semen with unsexed and double-dose sexed semen on Holstein Friesian Crossbred. A total of 100 Holstein Friesian crossbred dairy housed on farmers’ farms in the Pujon sub-district, Malang district. The cows were divided into two treatments consisting of 50 cows, including AI with unsexed semen (T1) and sexed semen (T2). The semen used was frozen semen with X-chromosomes bearing sperm that were separated using the Percoll density gradient centrifugation method. The fetal sex was observed using ultrasonography (USG) 50–90 days after insemination. The parameters observed were pregnancy rate (PR), percentage of fetal sex, and proportion of sperm. The results showed that the pregnancy rate of T1 was 50% and T2 was 52%, respectively. In addition, the female fetal of T1 was 77.78%, while T2 was 81.25%. Furthermore, the X and Y sperm proportions in frozen semen sexed X-bearing chromosomes were 77.9% and 22.1%, respectively. At the same time, the proportion of spermatozoa X and Y in non-sexed semen was 49.7% and 50.3%, respectively. To sum up, the proportion of female fetal by artificial insemination using sexed semen is higher than unsexed semen, with 81.25% and 77.78%, respectively.
