ABSTRACT
Objectives: to analyze the rosella petals mouthwash formulation as therapeutic antibiofilm agent on A.actinomycetemcomitans. Methods: This study was an experimental laboratory with post-test only control group design. A.actinomycetemcomitans was incubated for 24 hours, crystal violet staining assay of rosella petals mouthwash formulation concentration (1.56%, 3.125%, 6.25%, 12.5%, 25%), Chlorhexidine (CHX) 0.2 % as positive control, distilled sterile water as negative control. Results: One-way ANOVA showed that rosella petals mouthwash formulation could significantly reduce mature A. actinomycetemcomitans biofilm mass than negative control. Post Hoc test showed the concentration of 25% and 12.5% rosella petal mouthwash could potentially reduce mature A. actinomycetemcomitans biofilm mass compared to CHX 0.2% (p<0.05). Conclusions: Rosella petals mouthwash formulation showed significant reduction on mature A. actinomycetemcomitans biofilm mass.
