ABSTRACT
Objective: This study aimed to evaluate the effectiveness of Matricaria chamomilla (chamomile) ethanol extract as an antibiofilm agent against Porphyromonas gingivalis, a key pathogen in periodontal disease. Methods: A laboratory experimental study was conducted using P. gingivalis ATCC 33277. The antibiofilm activity of chamomile ethanol extract at concentrations of 10, 20, and 40 µg/mL was tested using a microtiter plate biofilm assay. Optical density (OD) readings were recorded to assess biofilm formation. Results: The 20 µg/mL concentration demonstrated the highest antibiofilm effectiveness with an OD of 0.280, compared to the positive control (chlorhexidine) at 0.216 and the negative control at 0.779. The 10 µg/mL and 40 µg/mL concentrations had higher OD values of 0.341 and 0.458, respectively. Conclusion: The 20 µg/mL concentration of chamomile extract was the most effective in inhibiting P. gingivalis biofilm formation, likely due to an optimal concentration of active compounds with antibiofilm activity.
