ABSTRACT
Objectives: To investigate the potential antibacterial activity of combining Hylocereus polyrhizus peel and Spatholobus littoralis stem against Staphylococcus aureus and Escherichia coli. Method: The well diffusion and plate count methods were used to test the combined extract's (1:1) antibacterial activity against Staphylococcus aureus and Escherichia coli. The following concentrations of the extract were prepared: 3.125%, 6.25%, 12.5%, 25%, 50%, 75%, and 100%. In this study, aquadest served as the negative control, and ciprofloxacin 50µg/mL as the positive control. Result: The extract formed inhibition zones against both bacteria. The MIC of the extract was determined to be 6.25%, while the MBC was 100%, indicating a bacteriostatic effect at 6.25%–50%, and a bactericidal effect at 100%. Overall, the extract showed a stronger antibacterial effect against Staphylococcus aureus than Escherichia coli. Conclusion: A combined extract of Hylocereus polyrhizus peel and Spatholobus littoralis stem has antibacterial activity against Staphylococcus aureus and Escherichia coli.
