ABSTRACT
Objectives: to discover the bio active compounds of B. flabellifer L. seed coat ethanol extract and examine its potential to inhibit the migration of HSC-3 cells. Methods: A colorimetric assay was used to determine the bio active compounds of the ethanol extract of B. flabellifer L. seed coat. This study involved seven groups: five treatment groups with extract concentrations of 93.75, 187.5, 375, 750, and 1500 µg/mL, a negative control (0.1% DMSO in culture medium), and a positive control (doxorubicin). Cell migration was assessed using a wound healing assay, with wound closure percentage as the outcome measure. Results: Colorimetric analysis confirmed the presence of phenolics, flavonoids, terpenoids, tannins, and alkaloids in the B. flabellifer L. seed coat ethanol extract. The extract inhibited HSC-3 cell migration, with the greatest effect observed at 750 µg/mL, showing the lowest wound closure. Conclusion: The extract demonstrates anticancer potential in OSCC by suppressing cell migration.
