ABSTRACT
Objectives: Natural ingredients are increasingly explored as alternative antibacterial agents. Plectranthus amboinicus leaves, traditionally used as herbal medicine. Periodontitis is a common inflammatory oral disease that caused by bacteria such as Fusobacterium nucleatum and Treponema denticola. Alongside therapeutic treatment, plaque control through brushing and mouthwash is crucial. Method: This study evaluated the antibacterial activity of ethanol, ethyl acetate, and hexane fractions of P. amboinicus leaf extract at concentrations of 25%, 50%, and 75%, using the agar diffusion method with chlorhexidine as a positive control. Result: The ethanol extract and hexane fraction at 75% concentration demonstrated significant antibacterial activity, effectively inhibiting the growth of T. denticola and F. nucleatum with inhibition zones measuring 13.93 mm and 14.68 mm, respectively, showing a significant difference compared to the negative control. Conclusion: These results suggest that P. amboinicus leaf extract, particularly in ethanol and hexane fractions, possesses effective antibacterial properties against key periodontal pathogens.
