ABSTRACT
The use of animals has been considered an essential part of biomedical research. Animals are also used in the safety and efficacy testing of drugs and other means of preventing or treating those diseases or traumatic insults to the body. The welfare of animals used for scientific experiments is in most countries protected by strict laws and regulations dictating the extent of research that can be performed. The lack of a proper randomization protocol is an important source of bias. Many animal studies do not report the randomization method or even whether it was used. The selection of the appropriate statistical test is extremely important for the outcome of the study. An important difference between the test animal and the patient is the health background and age. The experimental model is usually young and healthy whereas the patient is often older and with an underlying disease condition.
