ABSTRACT
Sperm sexing as several problems, such as decreased sperm motility, damage to cell membranes, and the high price of Bovine Serum Albumin (BSA). A study is needed to reduce the risk of sperm damage and sperm capacity using cheap albumin. This study aimed to determine sperm quality, the proportion of X and Y sperm using the albumin column method with Ophiocephalus striatus fish albumin extract (OSAE). Semen was collected using an artificial vagina from three Brahman cross bovine. Ophiocephalus striatus fish albumin extract, albumin columns were made in different concentrations according to P0 BSA (5% top :10% bottom), P1 OSAE (2% top: 4% bottom), P2 OSAE (3% top: 5% bottom), dan P3 OSAE (4% top: 6% bottom), P4 (7% top: 9% bottom). The parameters observed included sperm motility, sperm viability, sperm abnormalities, X and Y sperm proportions. Analysis was carried out using one-way analysis of variance (ANOVA). The results showed that the proportion of X and Y sperm using BSA 5%: 10% (67% : 73.5%) was higher (P<0.05) compared to OSAE 2%: 4% (39.20% : 75.50%), OSAE 3%: 5% (50.87%: 73.20%), OSAE 4%:6% (61.25%: 68.60%) and OSAE 7%:9% (64.64%: 66.78%). Post-thawing sperm motility and viability of P1 were highest (67.60%) than other treatments. Sperm viability (74.61%) and sperm abnormalities in P4 were highest (8.7%) than those of other treatments. In conclusion, the use of OSAE 4%:6% effective for separating the highest proportion of X and Y sperm, and OSAE 2%:4% produce the highest sperm motility and sperm viability after freezing.
