ABSTRACT
Phyllanthus niruri (meniran) is well known as an Indonesian traditional medicine due to its antioxidant properties to treat several infectious diseases. Meniran extract is a potential candidate for feed additives to substitute antibiotics. However, the biological activity of meniran has remained to be fully elucidated. The present study aimed to evaluate the anti-inflammatory effects of a meniran extract (ME) on lipopolysaccharide-induced RAW264.7 macrophage cells and were investigated via DPPH radical scavenging assay, CCK-8 assay, NO-assay, and RT-PCR analysis. LPS-induced RAW264.7 macrophage cells were grown in RPMI media containing 10% FBS, and 1% penicillin-streptomycin and then were cultured with ME (25, 50, 100, 200, and 400 ppm). ME showed an anti-inflammatory by increasing cell proliferation and was not toxic up to 400 ppm (P < 0.05). The ME activity suppressed the production of nitric oxide (NO) and the mRNA expression of inducible NO synthase. It also decreases the production of the pro-inflammatory cytokine's interleukin-6, Nuclear factor-κB, and tumor necrosis factor-α in mRNA expression. These results suggest that ME has anti-inflammatory properties that can be used to prevent or cure inflammatory diseases and potential candidate for feed additives to substitute antibiotics.
