ABSTRACT

This chapter explores the microbial and compositional properties of 25 oil and sludge samples collected from crude pipelines. To achieve this, the authors employed various analytical techniques, including: (a) quantitative polymerase chain reaction (qPCR) to assess microbially influenced corrosion (MIC) in the oil and sludge samples; (b) spectroscopy to analyze the morphology and elemental composition of sludge samples using environmental scanning electron microscopy-energy dispersive X-ray spectroscopy (ESEM-EDS) and the water fraction separated from the sludge using inductively coupled plasma optical emission spectrometry (ICP-OES); and (c) diffraction techniques, including quantitative phase analysis of the crystalline components in sludge deposits using high-resolution X-ray powder diffraction (XRD) data and the Rietveld method.