ABSTRACT
Objectives: The aim of this study was to analyze the preventive antibiofilm activity of rosella petals mouthwash formulation on Aggregatibacter actinomycetemcomitans in vitro. Methods: Phytochemical tests were done to identify active substances. Biofilm assay was used to analyze biofilm growth with different concentrations (1.56%, 3.125%, 6.25%, and 12.5%) of rosella petals mouthwash formulation. Result: The rosella petals mouthwash formulation contains active compounds of flavonoids, saponins, tannins, and polifenol. The biofilm assay results showed that all rosella concentrations exhibited preventive antibiofilm activity to Aggregatibacter actinomycetemcomitans compared with a negative control (p <0.05). Conclusion: Rosella petals mouthwash formulation were effective in inhibiting biofilm growth of Aggregatibacter actinomycetemcomitans. Rosella petals mouthwash formulation might be useful as an adjunctive therapy for periodontal supportive care. Future studies are still needed to confirm this result in vitro to analyze the efficacy of rosella petal mouthwash formulation.
