ABSTRACT
Objectives: This study aims to determine the effect of Plectrantus amboinicus leaf nanoparticle extract on the growth inhibition zone of Treponema denticola, Fusobacterium nucleatum, and Enterococcus faecalis. Methods : The P. amboinicus leaf extract ethyl acetate fraction of 12.5%, 25%, and 50% against the bacteria T. denticola, F. nucleatum, and E. faecalis which were incubated for 24 hours on MHA media using the paper disc diffusion method with NaOCl and chlorhexidine as positive controls. Results : The phytochemical test P. amboinicus leaf extract ethyl acetate fraction results showed active compound of phenolics, flavonoids, steroids, terpenoids, saponins, tannins, and alkaloids. The diameter of inhibition zone obtained from P. amboinicus leaf extract ethyl acetate fraction increased with increasing concentration. Conclusion: The ethyl acetate fraction of P. amboinicus leaf extract has inhibitory power in inhibiting the growth of T. denticola, F. nucleatum, and E. faecalis.
