ABSTRACT
Objectives: To determine the effect of red betel leaf extract (RBLE) in inhibiting Streptococcus sanguinis biofilm formation. Methods: Laboratory experimental with a quasi-experimental research design in four test and two control groups. The test group consisted of RBLE concentrations of 25%, 50%, 75% and 100%. The control group was chlorhexidine 0.2% as a positive control and dimethyl sulfoxide (DMSO)1% as a negative control. Inhibition of biofilm formation was measured using the Microtiter Plate Biofilm Assay method with a wavelength of 595 nm. Statistical analysis was performed using the Mann Whitney test. Results: The highest biofilm inhibition was found in 50% concentration, with an inhibition percentage of 22%. This result was still lower than the 0.2% chlorhexidine with an inhibition percentage of 31% but no significant differences. This inhibition may be due to the presence of compounds that function as antibacterial agents. Conclusion: RBLE has anti-biofilm activity against Streptococcus sanguinis.
