ABSTRACT
Asiaticoside, an antioxidant compound derived from the Gotu Kola (Centella asiatica (L.) Urb) plant, has a high molecular weight, limiting its ability to penetrate the skin. This study aims to evaluate the effects of adding Carbopol, propylene glycol, and their combination on the characteristics and penetration rate of Centella asiatica leaf extract gel formulations. Four gel variations were prepared: F1 (without Carbopol and propylene glycol), F2 (without propylene glycol), F3 (with both Carbopol and propylene glycol), and F4 (without Carbopol). The physical properties of these formulations were assessed through organoleptic, homogeneity, pH, viscosity, rheology, spreadability, and mechanical tests. Penetration tests were performed using a Franz Diffusion Cell with Whatman No. 1 filter paper soaked in Spangler's fluid, and the results were analysed with LC-MS/MS. Findings revealed that Carbopol increased viscosity and reduced spreadability, while propylene glycol decreased both viscosity and spreadability. The penetration tests showed that incorporating an enhancer significantly improved Asiaticoside penetration, with F3 demonstrating the highest rate (F3 > F2). This study suggests combining Carbopol and propylene glycol in gel formulations effectively enhances Asiatioside penetration, offering the potential for more effective topical wound healing treatments.
