ABSTRACT
Microparticle image velocimetry was developed by J. G. Santiago et al. to investigate microscale flow field, and it soon became one of the most versatile experimental tools in the field of microfluidic research. Optical diagnostics has played a significant role in experimental fluid mechanics, beginning with the development of schlieren and shadowgraphy imaging of compressible flows in the early twentieth century. Laser Doppler velocimetry has been a standard optical measurement technique in fluid mechanics since the 1970s. The random Brownian displacements cause particle trajectories to fluctuate about the deterministic path lines of the fluid flow field. The beam coming out the objective lens illuminates a large volume of fluid within the microchannel in which the seeding particles are suspended. Medical and biological fluid dynamic researches require a nonintrusive technique that has both spatial and temporal resolving power. The fluid was then injected into the gap between the two glass plates, and the several particle images were captured.
