ABSTRACT
This chapter describes a generalized system for routine evaluation of lung injury with reference to more complicated procedures that have been employed by investigators to answer specific questions. It outlines some of the standard procedures used to quantitate these events. A description of methods for establishing models of lung injury based on ozone exposures is therefore presented with reference to and discussion of procedures and assays useful to investigators interested in using ozone for the study of lung injury. Animals should be kept for up to two weeks in high-efficiency particulate-filtered air, isolated from other batches of animals to prevent possible cross-infection before exposure. Relatively simple procedures have been developed for early detection of pulmonary injury based on the measurements of enzymes and protein in bronchoalveolar lavage fluid. The brief review has demonstrated how ozone exposure of experimental animals can provide useful models of lung alveolar and terminal bronchiolar injury that are well characterized by both biochemical and histological methods.
