ABSTRACT
I. Radioactive Labeling Methods .............................................................................................68 A. Nick-Translation Labeling of dsDNA.......................................................................68 B. Random-Primer Labeling of dsDNA........................................................................71 C. Labeling of ssDNA ...................................................................................................72 D. 3
¢
-End Labeling to Fill Recessed 3
¢
Ends of dsDNA ..............................................72 E. 3
¢
-End Labeling of ssDNA with Terminal Transferase............................................74 F. 5
¢
-End Labeling Using Bacteriophage T4 Polynucleotide Kinase ..........................74 G. Labeling of RNA by
In Vitro
Transcription .............................................................75 Reagents Needed for Radioactive Labeling .........................................................................77
II. Nonradioactive Labeling Methods........................................................................................79 A. Random-Primer Labeling of dsDNA........................................................................80 B. Nick-Translation Labeling of dsDNA with Digoxigenin-11-dUTP.........................81 C. 3
