ABSTRACT
A quantitative analysis can only be successfully carried out if the substances of interest are adequately resolved. That is, the peaks must be separated sufficiently to allow the unique area or height of each peak to be accurately measured or calculated. This is necessary irrespective of whether the measurements are to be made manually on the chart paper or be calculated from acquired data by a computer. It follows that the degree of resolution that is necessary to achieve adequate quantitative accuracy needs to be addressed. In addition, despite chromatographic optimization, some components may still be only poorly resolved and, as a consequence, some action must be taken to access the peak areas and heights by special techniques. First, the conditions necessary to achieve adequate resolution will be considered.
