ABSTRACT

Pre-hybridization solution containing 50 mL 20× SSC and 130 mL HPLC-grade water

2. Next day, switch on 42°C shaking water bath and then dissolve in pre-hybridization solution while stirring:

• BSA (fraction V) 0.2 g

10% SDS 2 mL

• Make up volume to 200 mL with HPLC-grade water

Note: it is important to add the components in the order they are listed to avoid precipitation.