ABSTRACT

True to type mass propagations were attempted using nodal explant and shoot tip meristems in rose apple, a nutritionally rich fruit of India. For shoot multiplication from nodal explant and shoot tip, best response was in 4mg/l BAP in MS or ½ MS and 3mg/l kinetin in ½ MS respectively. Shoot multiplication from nodal explant took lesser period as compared to that of shoot tips. Rooting in shootlets of nodal explant recorded in 6mg/l IBA in half MS while shootlets derived from shoot tips induced roots in 7mg/l IBA in MS. In vitro raised plantlets from nodal explant took almost 90 days but 120 days from shoot tips. 1year conserved plantlets compare to immediately raised, recorded higher level of soluble protein content and peroxidase but lower catalase, depicting a variable pattern during growth and development. DNA fingerprinting using RAPD and ISSR markers did not show any DNA polymorphism, confirming the genetic stability.