ABSTRACT
Biofilm are the colony of sessile group of organisms that remain adhered to biotic and abiotic surface with the help of pilli and Extracellular Polymeric Substances (EPS). This is composed of carbohydrates, protein, nucleic acids and several other minerals that not only provides nutrition but also prevents the penetration of antibiotics and other drugs rendering antibiotic resistance. The overuse of antibiotics has resulted in the development of genotypic changes among the organism which has also been a great concern to fight against the resistant group of organisms. Thus our re-search focuses on antibiofilm activity of Zingiber officinale (Tuber) and to compare its efficacy with Azithromycin on the biofilm producing organisms isolated from pharyngeal throat infection. The organism obtained were characterised by 16S rRNA sequencing followed by sequence homology and the organism was identified to be Bacilliusparamycoides. The ethanolic extract of Zingiberofficinale showed Minimum Inhibitory Concentration (MIC) of 0.2514 µg/ml and its bioactive compounds showed inhibition of the viable cells of the organism at a greater extent than azithromycin. This was further studied with Scanning Electron Microscopy (SEM) which also showed that maximum reduction of the biofilm forming cells occurred due to the challenge of the ethanolic extract of Zingiberofficinale. The binding energy obtained from in-silico studies showed that the bioactive compounds tightly bind with the biofilm forming protein and thus inhibits the further proliferation of biofilm. Thus the present study concludes that the phytocompounds has efficiency to act against the biofilm forming bacterial cells to which antibiotic fails to act.
